Fig. 4

In vitro triacylglycerol (TAG) synthesis from stage 4 seed (S4) and the caruncle (C4) microsomal proteins, incubated with (a, c) [¹⁴C]-G3P and oleoyl-CoA or (b, d) [¹⁴C]-G3P and ricinoleoyl-CoA. Error bars show the standard deviations from triplicate assays for 30 or 60 min. DAG diacylglycerol, FFA free fatty acid, G3P glycerol-3-phosphate, GPAT glycerol-3-phosphate acyltransferase, LPA lysophosphatidic acid, LPC lysophosphatidylcholine, LPCAT lysophosphatidylcholine acyltransferase, MAG monoacylglycerol, PA phosphatidic acid, PC phosphatidylcholine, RA ricinoleic acid, TAG triacylglycerol