Proteins | Total protein (mg) | Specific activity (unit/mg) | Total activity (unit) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Cell lysatea (all proteins of E. coli contained EP) | 3.25 ± 0.39 | 0.08 ± 0.004 | 0.263 ± 0.06 | ||
Cell lysate with expression of BglucHb(all proteins of E. coli contained BglucH) | 6.63 ± 0.53 | 5.75 ± 0.042 | 37.59 ± 0.89 | ||
BglucHb in cell lysate | 6.63 ± 0.53 | 5.67 ± 0.35* | 37.33 ± 0.78 | 100.0 | 1.0 |
BglucLEHc in cell lysate (all proteins of E. coli contained BglucLEH) | 5.23 ± 0.34 | 6.07 ± 0.46 | 31.32 ± 0.65 | ||
BglucLEHc in cell lysate | 5.23 ± 0.34 | 5.99 ± 0.54* | 31.06 ± 0.63 | 100.0 | 1.0 |
Purified BglucH by Ni-NTA resin | 0.41 ± 0.09 | 68.85 ± 1.19 | 28.23 ± 0.51 | 75.62 | 12.14 |
Purified BglucLEH by Ni-NTA resin | 0.36 ± 0.04 | 68.22 ± 1.27 | 24.56 ± 0.59 | 79.07 | 11.39 |
Purified BglucLEH by one round of ITC | 0.32 ± 0.05 | 75.50 ± 1.54 | 24.16 ± 0.68 | 77.78 | 12.60 |
Purified BglucLEH by two rounds of ITC | 0.27 ± 0.03 | 76.22 ± 1.43 | 20.58 ± 0.47 | 66.26 | 12.72 |