Cell lysatea (all proteins of E. coli contained EP)
|
3.25 ± 0.39
|
0.08 ± 0.004
|
0.263 ± 0.06
| | |
Cell lysate with expression of BglucHb(all proteins of E. coli contained BglucH)
|
6.63 ± 0.53
|
5.75 ± 0.042
|
37.59 ± 0.89
| | |
BglucHb in cell lysate
|
6.63 ± 0.53
|
5.67 ± 0.35*
|
37.33 ± 0.78
|
100.0
|
1.0
|
BglucLEHc in cell lysate (all proteins of E. coli contained BglucLEH)
|
5.23 ± 0.34
|
6.07 ± 0.46
|
31.32 ± 0.65
| | |
BglucLEHc in cell lysate
|
5.23 ± 0.34
|
5.99 ± 0.54*
|
31.06 ± 0.63
|
100.0
|
1.0
|
Purified BglucH by Ni-NTA resin
|
0.41 ± 0.09
|
68.85 ± 1.19
|
28.23 ± 0.51
|
75.62
|
12.14
|
Purified BglucLEH by Ni-NTA resin
|
0.36 ± 0.04
|
68.22 ± 1.27
|
24.56 ± 0.59
|
79.07
|
11.39
|
Purified BglucLEH by one round of ITC
|
0.32 ± 0.05
|
75.50 ± 1.54
|
24.16 ± 0.68
|
77.78
|
12.60
|
Purified BglucLEH by two rounds of ITC
|
0.27 ± 0.03
|
76.22 ± 1.43
|
20.58 ± 0.47
|
66.26
|
12.72
|