Fig. 5

Effect of temperature and pH on the activity of purified recombinant Cel5A-h11 and Cel5A-h28. a Temperature effect when tested at the optimal pH 6.0. b Residual enzymatic activity at optimal pH and temperature after incubation at different temperatures (40 °C, 50 °C and 60 °C) for different duration (2, 5, 10, 20, 30, 40, 50 and 60 min). c pH effect when tested at the optimal temperature of each enzyme (40 °C for Cel5A-h11 and 50 °C for Cel5A-h28). d Residual enzymatic activity at optimal pH and temperature after 16 h incubation at 4 °C in different pH range of 3.0–10.0