Skip to main content

Table 2 Properties of the 30 cellulase candidates from the GH5 family used to determine their cellulolytic activity

From: Metatranscriptomics of the Hu sheep rumen microbiome reveals novel cellulases

ORFsa ID

Sample ID

Amino acid length

Best BLAST hit vs. NCBI (enzyme annotation)

Accession number

Identity %

Signal peptideb

c589026_g1_1

Cel5A-h1

344

Glycoside hydrolase

WP_022932641.1

70

N

c607167_g3_1

Cel5A-h2

556

Glycoside hydrolase

WP_020965515.1

49

Y

c350756_g1_5

Cel5A-h10

744

Glycoside hydrolase family 5 protein

WP_097035831.1

66

Y

c1499489_g1_2

Cel5A-h11

317

Glycoside hydrolase family 5 protein

WP_031559633.1

68

N

c676361_g1_1

Cel5A-h12

721

T9SS C-terminal target domain-containing protein

WP_101480019.1

52

Y

c423733_g1_1

Cel5A-h13

303

Glycoside hydrolase family 5 protein

WP_013498507.1

99

N

c7388_g1_1

Cel5A-h14

499

Endoglucanase

WP_115153074.1

57

N

c430069_g1_3

Cel5A-h15

512

Endoglucanase

CDC18601.1

52

N

c592373_g1_1

Cel5A-h16

633

Glycoside hydrolase family 5 protein

WP_084156318.1

55

Y

c581150_g3_2

Cel5A-h17

602

Putative carbohydrate-active enzyme

ADX05709.1

64

N

c602169_g11_1

Cel5A-h21

437

Cellulase

ADU86908.1

68

N

c535046_g1_1

Cel5A-h23

410

Glycoside hydrolase family 5 protein

WP_013499238.1

99

Y

c613169_g10_1

Cel5A-h24

335

Endoglucanase

WP_103869183.1

65

N

c660653_g1_1

Cel5A-h26

304

Hypothetical protein RASY3_16565

EXM37921.1

95

N

c602981_g3_1

Cel5A-h27

698

Glycoside hydrolase family 5 protein

WP_072812340.1

92

Y

c630880_g1_1

Cel5A-h28

480

Endoglucanase

CBL34359.1

50

Y

c533175_g1_1

Cel5A-h31

415

Cellulase

AHF24954.1

87

Y

c591423_g1_2

Cel5A-h32

609

Multispecies: hypothetical protein

WP_072418845.1

44

Y

c569332_g1_1

Cel5A-h35

617

Multispecies: glycosyl hydrolase family 5

WP_031534432.1

48

Y

c606942_g4_1

Cel5A-h37

731

Endoglucanase

WP_028517111.1

48

Y

c608068_g7_1

Cel5A-h38

343

Hypothetical protein

WP_044916478.1

68

N

c597031_g2_1

Cel5A-h41

445

Glycoside hydrolase family 5 protein

OUM69972.1

55

N

c573287_g1_1

Cel5A-h42

807

Endoglucanase

CDE12421.1

45

Y

c605251_g1_1

Cel5A-h44

387

Glycoside hydrolase family 5 protein

WP_014775421.1

38

N

c607602_g6_1

Cel5A-h45

593

Cellulose 1,4-beta-cellobiosidase

WP_037304596.1

99

N

c483261_g1_1

Cel5A-h47

365

Glycoside hydrolase family 5 protein

WP_080550482.1

72

N

c607437_g11_1

Cel5A-h49

577

Glycosyl hydrolase family 5

WP_013497304.1

99

N

c607437_g9_1

Cel5A-h50

690

Glycoside hydrolase family 5

WP_100068403.1

83

Y

c586094_g1_3

Cel5A-h53

736

T9SS C-terminal target domain-containing protein

WP_101480019.1

60

Y

c541302_g1_1

Cel5A-h54

433

Glycoside hydrolase family 5 protein

WP_083379902.1

65

Y

  1. aORFs, open reading frames
  2. bPresence (Y) of a signal peptide was predicted using the SignalP program