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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery

Fig. 4

Reduced birefringence upon treatment of catalytically delignified (CDL) poplar wood particles after low-temperature swelling in TFA and subsequent gelatinization. Relative crystallinity of cellulose as determined by darkfield microscopy (upper panels) compared to differential interference contrast microscopy (lower panels) for each treatment. WT = (Populus tremula × P. alba cv. INRA 717-1B4), high S-lignin = pAtC4H:AtF5H1, high G-lignin = pAtC4H:PtF5H2 RNAi, and high OH-G-lignin = p35S:PtCOMTa RNAi. Scale bar = 200 μm

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