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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Synechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant β-glucosidases

Fig. 1

Characteristics of expression cassettes and confirmation of integration of the pSyn_T7RNAP and pHN1_AMBGL17 vectors into the genome of Synechococcus elongatus PCC 7942. a pSyn_T7RNAP: Homologous recombination site (NSI a and NSI b), spectinomycin resistance gene (aadA), promoter region (PnrsB), T7 RNA polymerase, transcription termination region (rrnB). b pHN1_AMBGL17: Homologous recombination site (NSIII a and NSIII b), chloramphenicol resistance gene (cmlR), promoter region (PT7), β-glucosidase (AMBGL17), transcription termination region (rrnB). Dashed arrows indicate annealing position of the primers with respective sizes. c Confirmation of genomic integration by PCR. WT: S. elongatus wild type, NC: negative control reaction (without DNA template), 1 kb plus: 1 Kb Plus DNA Ladder

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Biotechnology for Biofuels and Bioproducts

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