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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Synechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant β-glucosidases

Fig. 3

Expression of the T7 system in Synechococcus elongatus PCC 7942. a Double-transgenic Synechococcus elongatus (PT7AMBGL17) prior to Ni2+ addition. Repressive protein is bound to the DNA and prevents endogenous RNA polymerase interaction with PnrsB that results in T7 RNA polymerase expression. b Mechanism of activation of the T7 system in S. elongatus PT7AMBGL17. After Ni2+ addition, this cation binds to the repressive protein, resulting in a change of conformation that blocks its interaction with DNA at the PnrsB region and results in T7RNA polymerase transcription by endogenous RNA polymerase. High levels of expression of T7 RNA polymerase, which action PT7 promoter and produces high levels of expression of the AMBGL gene. The thickness of the arrows indicates the intensity of transcription. c Relative expression of the T7 RNA polymerase (T7RNAP) and β-glucosidase (AMBGL17) genes in S. elongatus PT7AMBGL17. The level of gene expression was determined by qPCR, and results were normalized using the constitutive genes rnpB (R-subunit of ribonuclease P) and rpoD (Delta subunit of RNA polymerase). Different letters (uppercase: T7RNAP; lowercase: AMBGL17) show significant statistical differences (p < 0.05)

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