Table 2 Sequence of primers used in this work
PRIMERS | Forward primer (5′–3′) | Reverse primer (5′–3′) | Amplicon (bp) |
|---|---|---|---|
PCR | |||
T7RNAP | GGATCCGGAAGAGGCACTAAATGAACACG | CCAGGTACCGGAGTCGTATTGATTTGGCG | 2697 |
AMBGL17 | CACGAATTCTCGACGCTCTCCCTTATGCGACT | CTGACGCGTTACCGGAAGCAGTGTGACCGTGT | 2686 |
pHN1 | CATACGCGTAGGCGGTGAAGGGCAATCAGC | GTAGGAATTCAAGGGCACCAATAACT | 3850 |
Integration | |||
NSI | TGCTGCGTAACATCGTTGCTGCT | ATGTGATCGGAACCCTGAGCCGT | 1400 |
NSIII | TGGCAGAAGATCGTAGCGGCTCA | CGGATGAGCATTCATCAGGCGGG | 1850 |
qPCR | |||
RNPB | GTGAGGAGAGTGCCACAGAA | TAAGCCGGGTTCTGTTCTCT | 272 |
RPOD | GAGCAAGCAAGTCAGCGATTTG | TGAGCCCGCAACCACGATC | 224 |
T7RNAP | TTGTCCGGTCGAGGACATCCCTG | CTGATACGGCGAGACTTGCGAGC | 153 |
AMBGL17 | GAAAGGCGGTGGCTCTGTGGATG | GCGGCAGGATGTCACCTTCGTTT | 104 |