Fig. 2
Development of SecB random mutagenesis library and high-throughput screening. a Effect of Mn2+ concentrations on mutation rate of SecB. b Effect of butanol concentration on high-throughput screening. c High-throughput screening of SecB library containing 2800 variants. Red dot: 0.8% butanol stress, green dot: 1.0% butanol stress, purple dot: 1.2% butanol stress. Green dotted line: value of the WT SecB strain