Fig. 5

ITC result and consensus analysis SecB and homology proteins. a ITC result between SecB or SecBT10A and preMBP. N represents stoichiometric number. b Comparison of T10 and A10 in homotetramer of SecB. One subunit was depicted in green color, T10 and A10 were drawn in red and blue color, respectively, and substrate binding groove was illustrated with purple dotted square. c Multiple sequence alignment between SecB and homology proteins. Blue star: T10 site, green bar: residues interacted with SecA, yellow bar: residues involved in interaction with substrate. SecB homology proteins are from Escherichia coli (Accession No.: P0AG86), Klebsiella pneumoniae (A6TFK4), Serratia proteamaculans (A8GLB9), Vibrio mimicus (D2YLC5) Pseudomonas fluorescens (WP_060765829.1)