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Table 2 Tolerance of E. coli harboring SecB and SecBT10A toward diverse solvents

From: Enhancing butanol tolerance of Escherichia coli reveals hydrophobic interaction of multi-tasking chaperone SecB

Solventa logP E. coli JM109/pQE80L E. coli JM109/pQE80L-secB E. coli JM109/pQE80L-secBT10A
μb/h−1 Tc/% Ad/% μ/h−1 T/% A/% μ/h−1 T/% A/%
PBS 14 ± 1 15 ± 1 16 ± 1
DMF − 1.0 0.294 ± 0.015 42.4 ± 1.9 17 ± 1 0.462 ± 0.015 56.6 ± 1.1 17 ± 5 0.520 ± 0.014 65.0 ± 1.7 17 ± 3
Acetonitrile − 0.33 0.372 ± 0.014 35.4 ± 0.9 18 ± 2 0.511 ± 0.022 76.3 ± 1.7 18 ± 1 0.430 ± 0.012 76.9 ± 2.5 18 ± 2
Ethanol − 0.24 0.418 ± 0.011 51.1 ± 0.3 21 ± 8 0.420 ± 0.012 60.6 ± 1.0 23 ± 2 0.408 ± 0.013 64.3 ± 0.4 23 ± 1
Acetone − 0.23 0.100 ± 0.004 11.9 ± 0.4 16 ± 1 0.183 ± 0.013 14.3 ± 0.1 18 ± 6 0.114 ± 0.009 12.6 ± 0.7 18 ± 11
i-Propanol 0.39 0.121 ± 0.006 14.2 ± 0.4 23 ± 1 0.244 ± 0.012 20.0 ± 0.2 24 ± 3 0.251 ± 0.012 24.9 ± 0.2 26 ± 7
i-Butanol 0.76 0.083 ± 0.008 7.27 ± 0.28 13 ± 1 0.223 ± 0.011 13.1 ± 0.1 13 ± 5 0.235 ± 0.012 14.7 ± 0.2 12 ± 1
n-Butanol 0.88 0.019 ± 0.005 2.86 ± 0.40 14 ± 1 0.136 ± 0.016 9.13 ± 0.21 13 ± 1 0.169 ± 0.013 14.4 ± 0.9 11 ± 1
Toluene 2.5 0.016 ± 0.004 1.19 ± 0.18 1.9 ± 0.6 0.046 ± 0.008 4.83 ± 0.21 1.4 ± 0.8 0.071 ± 0.006 6.12 ± 0.21 1.3 ± 0.9
Cyclohexane 3.2 0.026 ± 0.006 0.59 ± 0.21 4.8 ± 1.1 0.067 ± 0.007 3.11 ± 0.23 3.5 ± 0.8 0.038 ± 0.005 2.27 ± 0.29 2.6 ± 1.9
  1. aSolvent concentration: 3% N,N-dimethylformamide (DMF), 3% acetonitrile, 3% ethanol, 1% acetone, 3% i-propanol, 3% i-butanol, 1.2% n-butanol, 0.2% toluene and 0.8% cyclohexane
  2. bμ denotes specific growth rate between 0 and 2 h (Eq. 1)
  3. cT denotes tolerance to butanol (Eq. 2)
  4. dA denotes adhesion rate of solvents (Eq. 3). All experiments were performed in triplicate. Growth curves of all the tested strains are available in Additional file