|
Solventa
|
logP
|
E. coli JM109/pQE80L
|
E. coli JM109/pQE80L-secB
|
E. coli JM109/pQE80L-secBT10A
|
|---|
|
μb/h−1
|
Tc/%
|
Ad/%
|
μ/h−1
|
T/%
|
A/%
|
μ/h−1
|
T/%
|
A/%
|
|---|
|
PBS
|
–
|
–
|
–
|
14 ± 1
|
–
|
–
|
15 ± 1
|
–
|
–
|
16 ± 1
|
|
DMF
|
− 1.0
|
0.294 ± 0.015
|
42.4 ± 1.9
|
17 ± 1
|
0.462 ± 0.015
|
56.6 ± 1.1
|
17 ± 5
|
0.520 ± 0.014
|
65.0 ± 1.7
|
17 ± 3
|
|
Acetonitrile
|
− 0.33
|
0.372 ± 0.014
|
35.4 ± 0.9
|
18 ± 2
|
0.511 ± 0.022
|
76.3 ± 1.7
|
18 ± 1
|
0.430 ± 0.012
|
76.9 ± 2.5
|
18 ± 2
|
|
Ethanol
|
− 0.24
|
0.418 ± 0.011
|
51.1 ± 0.3
|
21 ± 8
|
0.420 ± 0.012
|
60.6 ± 1.0
|
23 ± 2
|
0.408 ± 0.013
|
64.3 ± 0.4
|
23 ± 1
|
|
Acetone
|
− 0.23
|
0.100 ± 0.004
|
11.9 ± 0.4
|
16 ± 1
|
0.183 ± 0.013
|
14.3 ± 0.1
|
18 ± 6
|
0.114 ± 0.009
|
12.6 ± 0.7
|
18 ± 11
|
|
i-Propanol
|
0.39
|
0.121 ± 0.006
|
14.2 ± 0.4
|
23 ± 1
|
0.244 ± 0.012
|
20.0 ± 0.2
|
24 ± 3
|
0.251 ± 0.012
|
24.9 ± 0.2
|
26 ± 7
|
|
i-Butanol
|
0.76
|
0.083 ± 0.008
|
7.27 ± 0.28
|
13 ± 1
|
0.223 ± 0.011
|
13.1 ± 0.1
|
13 ± 5
|
0.235 ± 0.012
|
14.7 ± 0.2
|
12 ± 1
|
|
n-Butanol
|
0.88
|
0.019 ± 0.005
|
2.86 ± 0.40
|
14 ± 1
|
0.136 ± 0.016
|
9.13 ± 0.21
|
13 ± 1
|
0.169 ± 0.013
|
14.4 ± 0.9
|
11 ± 1
|
|
Toluene
|
2.5
|
0.016 ± 0.004
|
1.19 ± 0.18
|
1.9 ± 0.6
|
0.046 ± 0.008
|
4.83 ± 0.21
|
1.4 ± 0.8
|
0.071 ± 0.006
|
6.12 ± 0.21
|
1.3 ± 0.9
|
|
Cyclohexane
|
3.2
|
0.026 ± 0.006
|
0.59 ± 0.21
|
4.8 ± 1.1
|
0.067 ± 0.007
|
3.11 ± 0.23
|
3.5 ± 0.8
|
0.038 ± 0.005
|
2.27 ± 0.29
|
2.6 ± 1.9
|
- aSolvent concentration: 3% N,N-dimethylformamide (DMF), 3% acetonitrile, 3% ethanol, 1% acetone, 3% i-propanol, 3% i-butanol, 1.2% n-butanol, 0.2% toluene and 0.8% cyclohexane
- bμ denotes specific growth rate between 0 and 2 h (Eq. 1)
- cT denotes tolerance to butanol (Eq. 2)
- dA denotes adhesion rate of solvents (Eq. 3). All experiments were performed in triplicate. Growth curves of all the tested strains are available in Additional file
