Fig. 5
Stability of soluble and immobilized lipases. a Thermal stability of soluble PML, soluble PMLVG, Cry3Aa–PML and Cry3Aa–PMLVG. Reactions were heated for 1 h at various temperatures prior to measuring activity. Residual activity was normalized to the maximum activity for each respective construct. b Stability of soluble PML, soluble PMLVG, Cry3Aa–PML and Cry3Aa–PMLVG in MeOH. Soluble lipases and crystals were incubated in buffer only or 40% MeOH overnight and the residual activity was normalized to the activity after overnight incubation in buffer. All reactions were performed in triplicate and error bars were derived from the standard deviation of the mean