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Table 1 Kinetics of soluble and immobilized lipases

From: Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil

Construct

Activity (U/mg)

kcat (s−1)

KM (mM)

kcat/KM

(mM−1 s−1)

Soluble PML

63.9 ± 2.5

112 ± 12

1.77 ± 0.33

63.2 ± 0.21

Soluble PMLVG

75.5 ± 3.8

59.2 ± 2.9

0.234 ± 0.051

253 ± 0.22

Soluble DLZM4

106 ± 2.4

nd

nd

nd

Cry3Aa–PML

3.20 ± 0.34

5.89 ± 0.83

3.46 ± 0.71

1.70 ± 0.24

Cry3Aa–PMLVG

5.86 ± 0.24

3.48 ± 0.11

0.481 ± 0.048

7.24 ± 0.10

Cry3Aa–DLZM4

0.153 ± 0.028

nd

nd

nd

Cry3Aa*–PML

1.51 ± 0.066

nd

nd

nd

  1. One unit is defined as the amount of enzyme required to produce 1-μmol pNP per min at 25 °C. Measurements were performed in at least triplicate, and the averages are shown with the standard deviation of the mean
  2. nd not determined