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Table 1 Kinetics of soluble and immobilized lipases

From: Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil

Construct Activity (U/mg) kcat (s−1) KM (mM) kcat/KM
(mM−1 s−1)
Soluble PML 63.9 ± 2.5 112 ± 12 1.77 ± 0.33 63.2 ± 0.21
Soluble PMLVG 75.5 ± 3.8 59.2 ± 2.9 0.234 ± 0.051 253 ± 0.22
Soluble DLZM4 106 ± 2.4 nd nd nd
Cry3Aa–PML 3.20 ± 0.34 5.89 ± 0.83 3.46 ± 0.71 1.70 ± 0.24
Cry3Aa–PMLVG 5.86 ± 0.24 3.48 ± 0.11 0.481 ± 0.048 7.24 ± 0.10
Cry3Aa–DLZM4 0.153 ± 0.028 nd nd nd
Cry3Aa*–PML 1.51 ± 0.066 nd nd nd
  1. One unit is defined as the amount of enzyme required to produce 1-μmol pNP per min at 25 °C. Measurements were performed in at least triplicate, and the averages are shown with the standard deviation of the mean
  2. nd not determined