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Table 1 Overview of the different production rates obtained in this study

From: Efficient biochemical production of acetoin from carbon dioxide using Cupriavidus necator H16

Strain C. necator H16

Plasmid

Induction

mol acetoin/mol fructose

mol acetoin/mol CO2

mol H2/mol acetoin

ΔacoABC

pKRara-alsSD

 

0.06 ± 0.01

  

ΔacoABC

pKRara-alsSD

1.5 mM arabinose

0.87 ± 0.01

  

ΔacoABC

pKRrha-alsSD

 

0.03 ± 0.00

  

ΔacoABC

pKRrha-alsSD

1 mM rhamnose

0.89 ± 0.02

  

ΔacoABC

pKRphb-alsSD

 

0.26 ± 0.01

0.02 ± 0.00

432.69 ± 0.06

ΔacoABC_ΔphaC1

pKRphb-alsSD

 

0.13 ± 0.01

0.03 ± 0.01

346.68 ± 0.20

ΔacoABC_ΔphaC2

pKRphb-alsSD

 

0.65 ± 0.00

0.23 ± 0.01

31.86 ± 0.03

ΔacoABC_ΔphaC1_ΔphaC2

pKRphb-alsSD

 

0.63 ± 0.00

0.32 ± 0.00

23.18 ± 0.02

  1. Yields in mol acetoin/mol fructose (theoretical maximum 1 mol/mol) refer to the heterotrophic experiments while the yields in mol acetoin/mol CO2 (theoretical maximum 0.25 mol/mol) and mol H2/mol acetoin (theoretical minimum 10 mol/mol) were calculated from the experimental data of the autotrophic experiments