Fig. 4

Effect of DSA presoaking time on glucose yields from hydrolysis of pretreated solids by Accellerase 1500 cellulase at loadings of i 65 mg, ii 15 mg, iii 5 mg, and iv 2 mg cellulase protein/g glucan in unpretreated switchgrass. All DSA pretreatments were performed at 160 °C for 20 min with 0.5 wt% sulfuric acid to 7.5 wt% solid loadings of switchgrass knife milled to < 1 mm