Fig. 5

Effect of presoaking time on glucose yields from enzymatic hydrolysis of CELF pretreated by Accellerase 1500 cellulase at loadings of i 5 mg protein/g glucan in unpretreated switchgrass and ii 2 mg protein/g glucan in unpretreated switchgrass. All CELF pretreatments were performed at 150 °C for 25 min with 0.5 wt% sulfuric acid and a 0.889:1 THF/water mass ratio to 7.5 wt% solid loadings of switchgrass knife milled to < 1 mm