Fig. 6

Effect of milling (particle size) on enzymatic glucose yields for pretreated solids prepared by DSA at Accellerase 1500 cellulase loadings of i 65 mg, ii 15 mg, iii 5 mg, and iv 2 mg cellulase protein/g glucan in unpretreated switchgrass. All DSA pretreatments were performed at 160 °C for 20 min with 0.5 wt% sulfuric acid on switchgrass that had first been presoaked for 18 h at 4 °C at a 7.5 wt% solids loading