Fig. 7

Effect of milling (particle size) on glucose yields from CELF-pretreated solids for enzymatic hydrolysis with Accellerase 1500 at cellulase loadings of i 5 mg and ii 2 mg cellulase protein/g glucan in unpretreated switchgrass. All CELF pretreatments were performed at 150 °C for 25 min with 0.5 wt% sulfuric acid and a 0.889:1 THF/water mass ratio for switchgrass that had first been presoaked for 18 h at 4 °C and a 7.5 wt% solids loading