Table 1 Operating parameters of eight-stage countercurrent saccharification experiments
Train | 1 | 2 |
|---|---|---|
Substrate | α-Cellulose | α-Cellulose |
Added solids in every transfer (g) | 10 | 10 |
Added liquid in every transfer (mL) | 90 | 90 |
Enzyme loading (mg/g) | 2 | 5 |
Total reaction time (days) | 24 | 42 |
Transfer frequency | Every 48 h | Every 48 h |
Incubation temperature (°C) | 50 | 50 |
Enzyme-addition location (stage number) | 4 | 5 |