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Table 1 Top ten overexpression targets for increasing lipid production predicted by eMOMA-based design method

From: In silico identification of metabolic engineering strategies for improved lipid production in Yarrowia lipolytica by genome-scale metabolic modeling

Reaction abbreviation

Reaction description

EC number

Genes associated

Predicted yield improvement (%)

CITtam

Citrate/malate antiporter (mitochondrial)

n/a

YALI0F26323g

78.7

TRIGSY_GLC

Diglyceride acyltransferase

EC 2.3.1.20

(YALI0E16797g or YALI0E32769g)

74.0

ACCOACr

Acetyl-CoA carboxylase

EC 6.4.1.2

YALI0C11407g

67.7

FASn0COA

Fatty acyl-CoA synthase (n = 8, 10, 12, 14, 16, 18)

EC 2.3.1.86

(YALI0B15059g and YALI0B19382g and YALI0C11407g and YALI0E23185g)

38.9–56.7

CSm

Citrate synthase (mitochondrial)

EC 2.3.3.1

(YALI0E00638g or YALI0E02684g)

55.6

DESAT18

Stearoyl-CoA desaturase

EC 1.14.19.1

YALI0C05951g

35.4

ATPCitL

ATP:citrate lyase

EC 2.3.3.8

(YALI0E34793g and YALI0D24431g)

27.9

HSDxi

Homoserine dehydrogenase (NADH)

EC 1.1.1.3

YALI0D01089g

21.0

ASADi

Aspartate-semialdehyde dehydrogenase

EC 1.2.1.11

YALI0D13596g

21.0

ASPKi

Aspartate kinase

EC 2.7.2.4

YALI0D11704g

21.0