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Table 1 Biomass and starch productivity, CO2 bio-fixation rate, and amylose production of T. subcordiformis cultures with different amounts of NaHCO3 addition under nitrogen-depletion (−N) and nitrogen-limitation (±N) cultivation modes (mean ± SD, n = 3)

From: Application of an in situ CO2–bicarbonate system under nitrogen depletion to improve photosynthetic biomass and starch production and regulate amylose accumulation in a marine green microalga Tetraselmis subcordiformis

NaHCO3 (g L−1)

Cultivation mode

Biomass productivity (g L−1 day−1)

CO2 bio-fixation rate (g L−1 day−1)

Starch productivity (g L−1 day−1)

Amylose content (% total starch)

Amylose concentration (mg L−1)

Amylose productivity (mg L−1 day−1)

0

−N

0.29 ± 0.05α (2a)

0.55 ± 0.10α (2)

0.27 ± 0.02α (2)

27.8 ± 0.3α (2)

153 ± 8α (2)

54 ± 4α (2)

±N

0.74 ± 0.00A (8)

1.39 ± 0.00A (8)

0.46 ± 0.01A (8)

26.7 ± 1.5A (8)

984 ± 53A (8)

121 ± 7A (8)

0.2

−N

0.81 ± 0.10β (2)

1.52 ± 0.20β (2)

0.60 ± 0.01γ (2)

30.9 ± 0.5γ (2)

354 ± 2γ (2)

155 ± 1γ (2)

±N

0.74 ± 0.01A (8)

1.38 ± 0.02A (8)

0.47 ± 0.03AB (8)

26.7 ± 0.9A (8)

1011 ± 32AB (8)

125 ± 4AB (8)

1

−N

0.89 ± 0.03β (2)

1.67 ± 0.05β (2)

0.73 ± 0.02δ (2)

32.9 ± 0.3δ (2)

449 ± 16δ (2)

202 ± 8δ (2)

±N

0.82 ± 0.05B (8)

1.55 ± 0.10B (8)

0.51 ± 0.03B (8)

27.7 ± 1.0A (8)

1148 ± 108B (8)

142 ± 14B (8)

5

−N

0.80 ± 0.15β (2)

1.50 ± 0.29β (2)

0.41 ± 0.12β (2)

34.7 ± 0.9β (2)

276 ± 68β (2)

116 ± 34β (2)

±N

0.69 ± 0.05 (4)

1.29 ± 0.09 (4)

0.09 ± 0.04 (4)

59.3 ± 0.5 (4)

238 ± 83 (4)

56 ± 21 (4)

  1. The different Greek alphabets (α, β, γ and δ) represented significant difference (p < 0.05) between the cultures under −N cultivation mode. The different capital Latin alphabets (A and B) represented significant difference (p < 0.05) between the cultures under ±N cultivation mode
  2. aThe number in the parentheses represented the cultivation day used for calculation and comparison