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Table 3 Kinetic parameters of enzyme Tt_Thl and the variants

From: Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose

Enzyme

Km (mM) (acetyl-CoA as substrate)

Vmax (µmol/min/mg)

Thl

2.4 ± 0.6a

19.2 ± 3.2

Thl M1

3.3 ± 0.7

15.3 ± 4.3

Thl M2

2.6 ± 0.5

45.2 ± 5.2

  1. aError represents one standard deviation, n = 3