From: Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose
Organism | Strain | Description | Accession numbera | Source or reference |
---|---|---|---|---|
C. thermocellum | LL1004 | Wild-type C. thermocellum strain DSM 1313 | CP002416 | DSMZ |
AG929 | DSM1313 Δhpt ΔClo1313_0478 | SRP097241 | [8] | |
LL1644 | Strain AG929 Δldh with gene Tt_thl,Tt_hbd, Tt_crt, St_ter,Ts_bad and Ts_bdh integrated in the genome | SRP190757 | This work | |
LL1669 | Strain LL1644 ΔClo1313_1353-1356 | SRP190758 | This work | |
LL1668 | LL1669 with pLT_229 | SRP190756 | This work | |
E. coli | T7 Express lysY/lq | Used for heterologous protein expression | Â | New England Biolabs |
DH5α | Used for plasmid screening and propagation |  | New England Biolabs |