Fig. 5From: Inactivation of LACCASE8 and LACCASE5 genes in Brachypodium distachyon leads to severe decrease in lignin content and high increase in saccharification yield without impacting plant integrityTwo-photon fluorescence microscopy imaging and phloroglucinol–HCl staining of WT and lac5 lac8 lignified tissues. Cross sections of 30-day-old plants were imaged using two-photon fluorescence microscopy (green: 420–460-nm emission, purple: 495–540-nm emission a–d) or stained with phloroglucinol–HCl prior imaging under visible microscopy (e–i). WT: a, c, e, h; lac5 lac8: b, d, f, g, i. FF intrafascicular fibers, pXy protoxylem, mXy metaxylem, inFF interfascicular fibers, epi epidermis, mes mestome. Blue arrows show red staining of primary cell wall between interfascicular fiber cells in the double mutantBack to article page