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Table 4 Saccharification assays of extractive-free mature stem from Brachypodium wild type and laccase mutants

From: Inactivation of LACCASE8 and LACCASE5 genes in Brachypodium distachyon leads to severe decrease in lignin content and high increase in saccharification yield without impacting plant integrity

Genotype

Weight loss percentage

Glucose (mg g−1)

WT

22.4 ± 1.3

67.4 ± 2.6

lac5

28.1 ± 1.3*

97.9 ± 5.8*

lac8

20.4 ± 1.0

64.7 ± 0.4

lac5 lac8

48.8 ± 0.9*

160.1 ± 26.8*

  1. Saccharification is evaluated both by the weight loss percentage and by the amount of glucose released from the samples
  2. The data represent mean values and standard deviation from 3 to 4 biological replicates. Asterisks denote significant differences analyzed by one-way ANOVA (Tukey’s HSD, P < 0.05)
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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