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Table 2 Biomass hydrolysis profiles of enzymes and their blended preparations

From: A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains

Legend Enzyme % Saccharification (24 h) % Enhancement
Saturated cocktail Celluclast 1.5L (7 FPU) + BGL (21U) + Xylanase (500 U) 59.68 ± 2.38
Base cocktail Celluclast 1.5L (3.5 FPU) + BGL (21U) + Xylanase (500 U)
P” Base cocktail + P. funiculosum (3.5 FPU) 60.15 ± 3.44 0.47
P-11” Base cocktail + DBT-IOC-P-11 (3.5 FPU) 67.92 ± 1.72 8.24
P-11-9” Base cocktail + DBT-IOC-P-11-9 (3.5 FPU) 62.20 ± 0.32 2.52
P-11-10” Base cocktail + DBT-IOC-P-11-10 (3.5 FPU) 65.10 ± 0.53 5.42
P-11-15” Base cocktail + DBT-IOC-P-11-15 (3.5 FPU) 65.18 ± 0.21 5.5
P-11-22” Base cocktail + DBT-IOC-P-11-22 (3.5 FPU) 66.09 ± 0.38 6.41
P-11-31” Base cocktail + DBT-IOC-P-11-31 (3.5 FPU) 68.07 ± 1.84 8.39
P-11-33” Base cocktail + DBT-IOC-P-11-33 (3.5 FPU) 61.83 ± 2.38 2.15
P-11-37” Base cocktail + DBT-IOC-P-11-37 (3.5 FPU) 64.80 ± 1.61 5.12
NCIM 1171” Base cocktail + P. janthinellum (3.5 FPU) 61.36 ± 2.38 1.68
EMS” Base cocktail + DBT-IOC-EMS (3.5 FPU) 60.46 ± 1.07 0.78
EU1” Base cocktail + DBT-IOC-EU1 (3.5 FPU) 64.9 ± 2.90 5.22
EU4” Base cocktail + DBT-IOC-EU4 (3.5 FPU) 58.3 ± 2.95 ND
EUI-DU3” Base cocktail + DBT-IOC-EU1-DU3 (3.5 FPU) 60.5 ± 0.75 0.82
RC-30” Base cocktail + RC-30 (3.5 FPU) 66.25 ± 1.1 6.57
  1. ND enhancement was not detected
  2. * Fungal extract spiking was done in base cocktail which contains Celluclast 1.5L (3.5 FPU) + BGL (21U) + Xylanase (500 U) to maintain constant enzyme dosage in terms of filter paper units FPU (7 FPU/g biomass) in all experiments
  3. ” Symbol represents the blend of fungal extract (3.5 FPU) and Celluclast 1.5L (3.5 FPU) employed to degrade pretreated rice straw