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Table 2 Biomass hydrolysis profiles of enzymes and their blended preparations

From: A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains

Legend

Enzyme

% Saccharification (24 h)

% Enhancement

Saturated cocktail

Celluclast 1.5L (7 FPU) + BGL (21U) + Xylanase (500 U)

59.68 ± 2.38

Base cocktail

Celluclast 1.5L (3.5 FPU) + BGL (21U) + Xylanase (500 U)

P”

Base cocktail + P. funiculosum (3.5 FPU)

60.15 ± 3.44

0.47

P-11”

Base cocktail + DBT-IOC-P-11 (3.5 FPU)

67.92 ± 1.72

8.24

P-11-9”

Base cocktail + DBT-IOC-P-11-9 (3.5 FPU)

62.20 ± 0.32

2.52

P-11-10”

Base cocktail + DBT-IOC-P-11-10 (3.5 FPU)

65.10 ± 0.53

5.42

P-11-15”

Base cocktail + DBT-IOC-P-11-15 (3.5 FPU)

65.18 ± 0.21

5.5

P-11-22”

Base cocktail + DBT-IOC-P-11-22 (3.5 FPU)

66.09 ± 0.38

6.41

P-11-31”

Base cocktail + DBT-IOC-P-11-31 (3.5 FPU)

68.07 ± 1.84

8.39

P-11-33”

Base cocktail + DBT-IOC-P-11-33 (3.5 FPU)

61.83 ± 2.38

2.15

P-11-37”

Base cocktail + DBT-IOC-P-11-37 (3.5 FPU)

64.80 ± 1.61

5.12

NCIM 1171”

Base cocktail + P. janthinellum (3.5 FPU)

61.36 ± 2.38

1.68

EMS”

Base cocktail + DBT-IOC-EMS (3.5 FPU)

60.46 ± 1.07

0.78

EU1”

Base cocktail + DBT-IOC-EU1 (3.5 FPU)

64.9 ± 2.90

5.22

EU4”

Base cocktail + DBT-IOC-EU4 (3.5 FPU)

58.3 ± 2.95

ND

EUI-DU3”

Base cocktail + DBT-IOC-EU1-DU3 (3.5 FPU)

60.5 ± 0.75

0.82

RC-30”

Base cocktail + RC-30 (3.5 FPU)

66.25 ± 1.1

6.57

  1. ND enhancement was not detected
  2. * Fungal extract spiking was done in base cocktail which contains Celluclast 1.5L (3.5 FPU) + BGL (21U) + Xylanase (500 U) to maintain constant enzyme dosage in terms of filter paper units FPU (7 FPU/g biomass) in all experiments
  3. ” Symbol represents the blend of fungal extract (3.5 FPU) and Celluclast 1.5L (3.5 FPU) employed to degrade pretreated rice straw