Fig. 3

Optimal pH (a), pH stability (b), optimal temperature (c) thermostability (d) and thermal denaturation half-lives (e) of the purified PbRex8. Symbols for optimal pH and pH stability: citrate (open square, pH 3.0–6.0); acetate buffer (open diamond, pH 4.0–6.0); MES (open circle, pH 5.5–6.5); MOPS (open triangle, 6.5–7.5); Tris–HCl (filled square, pH 7.0–9.0); CHES (filled diamond, pH 8.0–10.0); Gly-NaOH (filled circle, pH 9.0–10.5); CAPS (filled triangle, 10.0–11.0). Thermal denaturation half-lives of PbRex8 were determined at 50 °C (filled square), 55 °C (filled circle) and 60 °C (filled triangle) for 8 h. The values are the average of experiments performed in triplicate