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Table 1 Substrate specificity of PbRex8 from P. barengoltzii CAU904

From: Biochemical characterization of a novel exo-oligoxylanase from Paenibacillus barengoltzii suitable for monosaccharification from corncobs

Substrate

Specific activity (U/mg)a

Relative activity (100%)

X2

67 ± 1

19.5

X3

331 ± 3

96.5

X4

343 ± 5

100

X5

237 ± 9

69.1

X6

177 ± 12

51.6

Birchwood xylan

2.2c

–b

Beechwood xylan

2.6c

–

Oat spelt xylan

1.3c

–

Oat β-glucan

1.3c

–

Barely β-glucan

1.0c

–

Lichenin

0.5c

–

  1. All data are mean values ± standard deviations of triplicate measurements
  2. aThe specific activity of PbRex8 was determined by measuring the enzyme’s activity in citrate buffer (pH 5.5) at 55 °C using various substrates. No enzyme activity was detected towards X3r, X4r, colloidal chitin, CMC, chitosan, locust bean gum and pNP-X
  3. bRelative activity < 1%
  4. cThe standard deviations < 0.1
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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