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Table 2 Substrate specificities of mutants

From: Biochemical characterization of a novel exo-oligoxylanase from Paenibacillus barengoltzii suitable for monosaccharification from corncobs

Substrate

Specific activity (U/mg)a

R67A

N122A

R253A

X2

2.5 ± 0.3

70 ± 0.9

72 ± 1.1

X3

276 ± 4

368 ± 8

356 ± 5

X4

292 ± 6

190 ± 4

178 ± 3

  1. aThe specific activity was determined by measuring the enzyme’s activity in 5 mM citrate buffer (pH 5.5) at 55 °C using X2, X3 and X4 as the substrates
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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