Table 2 List of genetic parts demonstrated in R. opacus, including plasmid backbones, selection markers, promoters, and recombination-related parts

Plasmid backbones

pAL5000 (short)

Other names: pXYLA and pNV18; ~ 11 copies per chromosome

[62, 73, 90,91,92,93,94]

pAL5000 (long)

Other names: pJAM2 and pJEM; ~ 3 copies per chromosome

[63, 73, 90, 93]

pNG2

Derived from Corynebeacterium spp.; ~ 10 copies per chromosome

[82, 84]

pGA1

Derived from Corynebeacterium spp.

[63, 87, 95, 96]

pSR1

Derived from Corynebeacterium spp.

[85, 86, 88]

pB264

Derived from Rhodococcus sp. B264; curable; ~ 8 copies per chromosome

[73]

Selection markers

Kanamycin

50 μg/mL (selection)

250 μg/mL (plasmid function maintenance)

[85, 88, 97]

Gentamicin

10 μg/mL

[82]

Spectinomycin

100 μg/mL

[82]

Thiostrepton

1 μg/mL

[97]

Chloramphenicol

34 μg/mL

[98, 99]

Hygromycin B

50 μg/mL

[73]

SacB

Negative selection; sensitizes cell to sucrose

[100, 101]

Promoters

pTipA

Inducible with thiostrepton

[97, 102, 103]

pAcet

5× inducible with acetamide

[98, 104]

pBAD

59× inducible with arabinose

[104]

pTet

67× inducible with anhydrotetracycline (aTc)

[104]

pLPD06740

247× inducible with phenol

[104]

pLPD06575

Inducible with phenol

[104]

pLPD06699

Up to 39× inducible with phenol, protocatechuic acid, sodium benzoate, 4-hydroxybenzoate, vanillate, and guaiacol

[104]

pLPD06568

Up to 80× inducible with phenol, sodium benzoate, and guaiacol

[104]

pLPD03031

18× repressible with ammonium

[104]

IGRI’ and IGRIV’

Inducible with 2,4-dinitrophenol (DNP)

[100]

Recombinases

Che9c60

GC-rich homologue of RecE

[73, 79, 105]

Che9c61

GC-rich homologue of RecT

[73, 79, 105]

Neutral sites

ROCI-2

R. opacus chromosomal locus

[73]

ROCI-3

R. opacus chromosomal locus

[73]

ROP8I-1

R. opacus endogenous plasmid 8 locus

[73]