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Table 2 List of genetic parts demonstrated in R. opacus, including plasmid backbones, selection markers, promoters, and recombination-related parts

From: Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds

Part type Name Properties/notes Source
Plasmid backbones pAL5000 (short) Other names: pXYLA and pNV18; ~ 11 copies per chromosome [62, 73, 90,91,92,93,94]
pAL5000 (long) Other names: pJAM2 and pJEM; ~ 3 copies per chromosome [63, 73, 90, 93]
pNG2 Derived from Corynebeacterium spp.; ~ 10 copies per chromosome [82, 84]
pGA1 Derived from Corynebeacterium spp. [63, 87, 95, 96]
pSR1 Derived from Corynebeacterium spp. [85, 86, 88]
pB264 Derived from Rhodococcus sp. B264; curable; ~ 8 copies per chromosome [73]
Selection markers Kanamycin 50 μg/mL (selection)
250 μg/mL (plasmid function maintenance)
[85, 88, 97]
Gentamicin 10 μg/mL [82]
Spectinomycin 100 μg/mL [82]
Thiostrepton 1 μg/mL [97]
Chloramphenicol 34 μg/mL [98, 99]
Hygromycin B 50 μg/mL [73]
SacB Negative selection; sensitizes cell to sucrose [100, 101]
Promoters pTipA Inducible with thiostrepton [97, 102, 103]
pAcet 5× inducible with acetamide [98, 104]
pBAD 59× inducible with arabinose [104]
pTet 67× inducible with anhydrotetracycline (aTc) [104]
pLPD06740 247× inducible with phenol [104]
pLPD06575 Inducible with phenol [104]
pLPD06699 Up to 39× inducible with phenol, protocatechuic acid, sodium benzoate, 4-hydroxybenzoate, vanillate, and guaiacol [104]
pLPD06568 Up to 80× inducible with phenol, sodium benzoate, and guaiacol [104]
pLPD03031 18× repressible with ammonium [104]
IGRI’ and IGRIV’ Inducible with 2,4-dinitrophenol (DNP) [100]
Recombinases Che9c60 GC-rich homologue of RecE [73, 79, 105]
Che9c61 GC-rich homologue of RecT [73, 79, 105]
Neutral sites ROCI-2 R. opacus chromosomal locus [73]
ROCI-3 R. opacus chromosomal locus [73]
ROP8I-1 R. opacus endogenous plasmid 8 locus [73]
  1. For promoters inducible with multiple chemicals, the highest published fold change for a single compound is reported