Development of Rhodococcus opacus as a chassis for lignin valorization and bioproduction of high-value compounds

Table 3 Bioproduction by R. opacus wild-type and engineered strains on various feedstocks

Strain	Substrate	Product	Production value	References
R. opacus PD630	glucose/glycerol (7:3)	Carotenoids and lipids	0.99 mg/L and 2.4 g/L, respectively	[116]
R. opacus PD630	Pre-treated corn stover	FAME	1.3 g/L	[60]
R. opacus PD630	Glycerol	TAGs	1.4 g/L, 38.4% DCW	[117]
R. opacus PD630 (engineered)	Glucose	Fatty acids	46% DCW	[120]
R. opacus MITXM-61 (engineered)	Corn stover hydrolysates	TAGs	15.9 g/L, 54% DCW	[115]
R. opacus MITGM-173 (evolved)	glycerol/ glucose/xylose (1:2:2)	TAGs	13.6 g/L, 51.2% DCW	[119]
R. opacus PD630	Crude whey	Fatty Acids	45.1% DCW	[121]
R. opacus PD630	Switchgrass pyrolysis oil	Lipid	pH 7: 0.078 g/L, 21.9% DCW pH 4: 0.066 g/L, 25.8% DCW	[52]
R. opacus PD630	Kraft lignin (+ laccase)	Lipid	0.145 g/L	[59]
R. opacus PD630	Olive mill waste	Lipid	~ 1.9 g/L, 80% DCW	[122]
R. opacus PD630 PVHG6	phenol/vanillate/4-hydroxybenzoate/guaiacol/benzoate (1:1:1:1:1)	Lipid	0.13 g/L, 44% DCW	[9]
R. opacus PD630 (engineered)	Gluconate and whey	Wax esters	Gluconate: 46% total neutral lipids Whey: NR	[99]
R. opacus PD630	Poplar lignin hydrolysis slurry	Lipid	NR	[34]