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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesei RUT-C30

Fig. 6

Cytosolic Ca2+ levels and the transcription level of the final calcium signaling gene crz1. a Analysis of cytosolic Ca2+ levels via the Ca2+ fluorescent probe Fluo-3/AM. T. reesei Δ76238 and parental strain RUT-C30 were cultured in MA medium for 96–120 h, using glucose as the sole carbon source. For detection, 50 μM Fluo-3/AM was used, and its intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents free cytosolic Ca2+. b Transcriptional level of the final calcium signaling gene crz1 was evaluated by quantitative real-time PCR (qPCR). T. reesei strains were grown using glucose for 96 or 120 h. The data were normalized to the expression of RUT-C30 over 96 h, with the sar gene used as an endogenous control in all samples. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences (***p < 0.001, Student’s t test)

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