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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesei RUT-C30

Fig. 7

Effects of Ca2+ channel inhibitor LaCl3 on growth, cytosolic Ca2+ concentration, and cellulase production. a Biomass dry weight of T. reesei RUT-C30 and Δ76238 was measured in 100 mL MA medium treated with different concentrations of LaCl3 for 120 h, using glucose as the sole carbon source. b PNPCase activity of Δ76238 was examined after culture in MA medium for 120 h containing 0, 0.25, 0.5, or 1 mM LaCl3. c Fluorescence analysis of LaCl3 influence on cytosolic Ca2+ burst in Δ76238. Δ76238 was cultured in MA medium for 120 h and treated with 0 or 0.5 mM LaCl3, using glucose as the sole carbon source. For detection, 50 μM Fluo-3/AM was used, and its intensity was monitored using the Nikon A1R confocal microscope. Green fluorescence represents the free cytosolic Ca2+. “a” in the figure indicates that conidia of Δ76238 cannot germinate with 1 mM LaCl3. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences (**p < 0.01, ***p < 0.001, Student’s t test)

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