Fig. 4

Strategy for CRISPR-Cas9/Cas9n mediated multiplex point mutations. a Assumed mechanism of CRISPR-Cas9/Cas9n mediated multiplex breaks. b Editing efficiency and CFU for multiplex point mutations using the CRISPR-Cas9/Cas9n system. In these genetic modifications, 500 bp homologous-arms were used to achieve recombination. All error bars represent the value of standard deviation which were caculated from three repeated experiments