Fig. 6

Strategy for improving CRISPR-Cas9/Cas9n mediated gene editing by regulating ligD. a Cell growths of strains with CRISPR-Cas9/Cas9n mediated gene editing under different induction conditions. Promoter P43 was used to overexpress ligD. The amyE was targeted for Cas9/Cas9n-induced genome cleavage in this study. b Editing efficiency for multiplex point mutations by the improved CRISPR-Cas9/Cas9n system. In these genetic modifications, 500 bp homologous-arms were used to achieve recombination. All error bars represent the value of standard deviation which were caculated from three repeated experiments