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Table 1 Sequences in RBS regions of the riboflavin operon genes of the 20 optimized strains

From: Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis

Strain

RBS region of ribB

RBS region of ribA

RBS region of ribH

CY23

ATATAAG

GTAAGAA

AAGAAGG

CY41

ATTAGAG

AAGGGGA

AGTGAGA

CY45

ATGGTGA a

AGGTGGA

ATGGGAA

CY43

AGACAGG

GGCTGGA

AGATGAG

CY47

AACATAG

AAGGAGG

ATAGAGA

CY124

AATGAGA

ACAAGGA

AGGATGG

CY79

AGTAAGA

AAAGGAG

GTAGGAA

CY6

ATGGTGA

---GAGGb

AGTAGAA

CY107

GGAGAGG

ATTGGAG

AAGAGGA

CY49

ATTGAGG

AGTCGAA

GTAAAAG

CY11

AGTCGAA

AAGGAGG

---GGAG

CY44

GGGAGAA

ATGTGAA

GGCTAGG

CY14

GGAGTAG

GATGGAA

AGTTGAG

CY46

AGTTGAG

AAAGAGA

AAGATAA

CY16

AATGGAG

AATGGAG

AGTTGAG

CY18

GCGAAGG

AGCGAGG

ATGGGAG

CY33

AGGGAGA

AATGGAG

GCGAAGG

CY24

GTTGAAG

AGGAGAA

AAGAAAG

CY17

AAAGAGG

ATTGGAG

AGCAGGA

CY15

AGGAGGA

AAGGAGG

AATGGAG

  1. aThe Wild type RBSs are shown in bold
  2. b“-” indicates base deletion in this location