Fig. 1

a Schematic illustration of the plasmid pX-DsRed (X = CEL3A, CEL7A, or CEL7B). Kan: kanamycin resistance; LB, left border of the binary vector; RB, right border of the binary vector; Pcbh, a modified CBH1 promoter; Ttrpc, Aspergillus nidulans trpC terminator; hyg, hygromycin B phosphotransferase gene. b Cellulase activities of T. reesei RUT-C30, RBGL, RCBH, RCMC, and RD grown on TMM + 2% cellulose for 5 days. pNPGase: the β-glucosidase activity; pNPCase: the CBH activity; CMCase: the CMC activity; FPase: the filter paper activity. Data are represented as the mean of three independent experiments and error bars express the standard deviations. c Observation of the localization of BGL-DsRed, CBH-DsRed, CMC-DsRed, and DsRed in T. reesei strains RBGL, RCBH, RCMC, and RD, respectively, grown on cellulose for 5 days by STED