Skip to main content
Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum

Fig. 1

Phenotype analysis of PoSet2 mutants. a Domain architecture analysis of PoSet2 orthologs. Referring to the websites SMART (http://smart.embl-heidelberg.de/) and Pfam database (http://pfam.xfam.org/), the maps were constructed with equal proportions of the respective sequences. A diagram of five conserved domains of Set2 orthologs: AWS, SET, PS, WW, and SRI domains. b Subcellular localization of PoSet2 in P. oxalicum. Upper left, white light; upper right, green fluorescence (green dots); bottom left, nuclear staining (blue dots); bottom right, merged (white dots). Yellow arrows indicating the location that showed green fluorescence and nuclear staining and the overlap of green dots and blue dots on the merged image (white dots). c Morphological characteristics of the colony on Vogel’s agar with 2% glucose, 1% starch, and 0.5% ball-milled microcrystalline cellulose. 1.5 μL of fresh spore solutions (107/mL) were spotted on Vogel’s medium ager plates with different carbon source, and then cultivated at 30 °C for 3–5 days. d Levels of conidiation on Vogel’s agar with 2% glucose. e Observation of mycelial growth and sporogenesis of WT and PoSet2 mutants. The length of the size bar shown in the figure represented an actual length of 20 μm. f Assay of the transcription level of brlA. Statistical significance tests were performed by one tailed, unequal variance t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page