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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Normal transcription of cellulolytic enzyme genes relies on the balance between the methylation of H3K36 and H3K4 in Penicillium oxalicum

Fig. 4

Methylation modification level of H3K4 and H3K36 toward the genes encoding prominent cellulolytic enzymes in WT and PoSet2 mutants. a The transcript abundance of seven genes encoding prominent cellulolytic enzymes assayed through qRT-PCR. b Analysis of the methylation modification level of H3K4 and H3K36 on the specific regions of target genes through ChIP-qPCR. Four genes were selected: two hemicellulase genes, namely, abf62A and xyn11A, and two cellulase genes, namely, cel6A/cbh2 and cel5B/eg2. The levels of H3K36me1, H3K36me3, and H3K4me3 of the target genes were assayed. The transcription start site (TSS) was designated as + 1. Six specific regions (R1 to R6) of each gene were designed for qPCR assay (top of each subgraph). Region 1 (R1) and region 2 (R2) were located upstream of the TSS. Region 3 (R3) covered the TATA-box and the initiator (Inr). Region 4 (R4) was located in the 5′ region of the CDS. Region 5 (R5) was located at the middle of the CDS, and region 6 (R6) was located in the 3′ region of the CDS. The values showed the means of the three biological replicates, and the error bar indicated standard deviation. Statistical significance tests were performed by one tailed, unequal variance t-test. *P < 0.05, **P < 0.01, ***P < 0.001

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