Fig. 5

Phenotypic analysis of PoSet1 mutants. a Domain architecture analysis of PoSet1 orthologs. b Subcellular localization of PoSet1 in P. oxalicum. Upper left, white light; upper right, green fluorescence (green dots); bottom left, nuclear staining (blue dots); bottom right, merged (white dots). c Morphological characteristics of the colony of 4-day-old cultures on Vogel’s agar with 2% glucose or 0.5% ball-milled microcrystalline cellulose. d Assay of the transcription level of brlA through RT-qPCR. e Activity assay of xylanase, arabinofuranosidase, pNPCase, and CMCase in WT and PoSet1 mutants. Enzyme activities were normalized to the ratio of corresponding biomass (IU/mg). Statistical significance tests were performed by one tailed, unequal variance t-test. *P < 0.05, **P < 0.01, ***P < 0.001. f Analysis of H3K4 methylation patterns through Western blot. Histone H3 was used as the loading control. The peak area and the data were obtained by the software Image J, representing the relative signal strength of the corresponding Western blot bands