Fig. 6

Methylation modification level of H3K4 and H3K36 toward genes encoding prominent cellulolytic enzymes in WT and PoSet1 mutants. a The transcript abundance of seven genes encoding prominent cellulolytic enzymes and four genes encoding TFs were assayed through qRT-PCR. The diagram on the left, three genes (xyn10A, xyn11A, and abf62A) encoding hemicellulases; the diagram in the middle, four genes (cel7A/cbh1, cel6A/cbh2, cel7B/eg1, and cel5B/eg2) encoding cellulases; the diagram on the right, four genes (creA, clrB, xlnR, and amyR) encoding TFs. b Analysis of the methylation modification level of H3K4 and H3K36 on the specific regions of target genes through ChIP-qPCR. Four genes were selected: two hemicellulase genes, namely, abf62A and xyn11A, and two cellulase genes, namely, cel6A/cbh2 and cel5B/eg2. The levels of H3K36me1, H3K36me3, and H3K4me3 of the target genes were assayed. The design of the six regions (R1–R6) of each gene locus is the same as the figure legend described in Fig. 4b. Relative enrichment of IP DNA was calculated as the percentage of the total input DNA. The values show the mean of the three biological replicates, and the error bar indicates standard deviation. Statistical significance tests were performed by one tailed, unequal variance t-test. *P < 0.05, **P < 0.01, ***P < 0.001