Fig. 2

Expression of heterologous GHs by recombinant G. thermoglucosidasius strains. The concentrated supernatants from wild type as well as recombinant G. thermoglucosidasius strains expressing GHs either CtCelA or Cel6B or CbCelA were separated in SDS-PAGE and extracellular heterologous GHs at the indicated time points (2–9 h) were detected by Western blotting using ANTI-FLAG M2 monoclonal antibody-horseradish peroxidise conjugate. WT represents concentrated supernatant from wild-type strain of G. thermoglucosidasius at 8 h; M represents the pre-stained protein ladder (10–250 kDa). Expected molecular mass of the proteins are indicated by red arrows. a Congo red-stained TSA-CMC agar plate streaked with recombinant G. thermoglucosidasius expressing CtCelA enzyme (zone C1, C2, C3) and wild-type strain (zone WT). b Western blot showing the 100-fold concentrated supernatants from recombinant strain expressing the 50 kDa CtCelA. c CMCase specific activity of extracellular fraction of recombinant G. thermoglucosidasius expressing CtCelA and wild-type G. thermoglucosidasius strains (WT). d Western blot showing the 100-fold concentrated supernatants from recombinant G. thermoglucosidasius expressing the 60 kDa Cel6B. e RACase specific activity of extracellular fraction of recombinant G. thermoglucosidasius expressing Cel6B and G. thermoglucosidasius wild-type (WT) strains. f Western blot showing the 200-fold concentrated supernatants of recombinant G. thermoglucosidasius expressing the 193 kDa CbCelA. g CMCase and h RACase specific activity of extracellular fraction of recombinant G. thermoglucosidasius CbCelA and wild-type G. thermoglucosidasius strains (WT). P values were calculated by student’s t test and results are shown as mean ± SEM of three biological replicates