Table 1 List of bacterial strains and plasmids used in this study. G. thermoglucosidasius is abbreviated as G. t. and E. coli as E. c
From: Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw
Strains/plasmids | Properties | References/source |
|---|---|---|
Strains | ||
 E. c. Top 10 | E. coli cloning strain | NEB |
 G. t. NCIMB 11955 | wild-type strain | TMO renewables |
 G. t. 11955 ∆pyrE | pyrE deletion strain of 11955 | Sheng et al. [26] |
 G. t. LS004 | ∆pyrE ∆ldh ∆pfl pdh up | Sheng et al. [26] |
 G. t. LS242 | ∆ldh ∆pfl pdh up | Sheng et al. [26] |
 G. t. ZB1b | Pldh::CtcelA; CtcelA expressed on plasmid constitutively; KanR | This study |
 G. t. ZB9a | Pldh:: cel6B; cel6B expressed on plasmid constitutively; KanR | This study |
 G. t. ZB6d | Pldh::CbcelA; CbcelA expressed on plasmid constitutively; KanR | This study |
 G. t. ZB3bInt | Pldh::cglT; cglT constitutively expressed in the chromosome at pyrE locus | This study |
 G. t. BZ9 | Pldh::CbcelA; CbcelA expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. ZB3bInt; KanR | This study |
 G. t. BZ10 | Pldh::CtcelA–cel6B; CtcelA–cel6B operon expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. ZB3bInt; KanR | This study |
 G. t. BZ242 | Pldh::cglT; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. LS242 | This study |
 G. t. BZ243 | Pldh::CbcelA; CbcelA expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. BZ242 strain; KanR | This study |
 G. t. BZ244 | Pldh::CtcelA–cel6B; CtcelA–cel6B operon expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. BZ242; KanR | This study |
Plasmids | ||
 pMTLgSlimS | G. thermoglucosidasius shuttle vector. RepB, ColE1, KanR | Sheng et al. [26] |
 pBSK–ldh | Synthesised ldh promoter | Biomatik |
 pBSK–CtcelA | Synthesised CtcelA gene | Biomatik |
 pBSK–cel6B | Synthesised cel6B gene | Biomatik |
 pBSK–cglT | Synthesised cglT gene | Biomatik |
 pBSK–CbcelA | Synthesised CbcelA gene | Biomatik |
 pMTLgSlimS–LS3 | G. thermoglucosidasius integration vector at pyrE locus | Sheng et al. [26] |
 pJ201–CtcelA | Biobrick vector harbouring CtcelA gene flanked by ldh promoter | This study |
 pMTLgSlimS–CtcelA | Expression vector for CtcelA with Pldh promoter | This study |
 pJ201–cel6B | Biobrick vector harbouring cel6B gene preceded by Pldh promoter | This study |
 pMTLgSlimS–cel6B | Expression vector for cel6B with Pldh promoter | This study |
 pJ201–CbcelA | Biobrick vector harbouring CbcelA gene flanked by Pldh promoter | This study |
 pMTLgSlimS–CbcelA | Expression vector for CbcelA with Pldh promoter | This study |
 pJ201–cglT | Biobrick vector harbouring cglT gene flanked by Pldh promoter | This study |
 pMTLgSlimS–cglT | Expression vector for cglT with Pldh promoter | This study |
 pMTLgSlimS–LS3–cglT | Integration vector for CglT with Pldh promoter at pyrE locus | This study |
 pMTLgSlimS–CtcelA–cel6B | Expression vector for CtcelA–cel6B operon with Pldh promoter | This study |