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Table 1 List of bacterial strains and plasmids used in this study. G. thermoglucosidasius is abbreviated as G. t. and E. coli as E. c

From: Engineering Geobacillus thermoglucosidasius for direct utilisation of holocellulose from wheat straw

Strains/plasmids Properties References/source
Strains
 E. c. Top 10 E. coli cloning strain NEB
 G. t. NCIMB 11955 wild-type strain TMO renewables
 G. t. 11955 ∆pyrE pyrE deletion strain of 11955 Sheng et al. [26]
 G. t. LS004 ∆pyrE ∆ldh ∆pfl pdh up Sheng et al. [26]
 G. t. LS242 ∆ldh ∆pfl pdh up Sheng et al. [26]
 G. t. ZB1b Pldh::CtcelA; CtcelA expressed on plasmid constitutively; KanR This study
 G. t. ZB9a Pldh:: cel6B; cel6B expressed on plasmid constitutively; KanR This study
 G. t. ZB6d Pldh::CbcelA; CbcelA expressed on plasmid constitutively; KanR This study
 G. t. ZB3bInt Pldh::cglT; cglT constitutively expressed in the chromosome at pyrE locus This study
 G. t. BZ9 Pldh::CbcelA; CbcelA expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. ZB3bInt; KanR This study
 G. t. BZ10 Pldh::CtcelAcel6B; CtcelAcel6B operon expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. ZB3bInt; KanR This study
 G. t. BZ242 Pldh::cglT; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. LS242 This study
 G. t. BZ243 Pldh::CbcelA; CbcelA expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. BZ242 strain; KanR This study
 G. t. BZ244 Pldh::CtcelAcel6B; CtcelAcel6B operon expressed on autonomous plasmid; cglT constitutively expressed in the chromosome at pyrE locus; derived from G. t. BZ242; KanR This study
Plasmids
 pMTLgSlimS G. thermoglucosidasius shuttle vector. RepB, ColE1, KanR Sheng et al. [26]
 pBSK–ldh Synthesised ldh promoter Biomatik
 pBSK–CtcelA Synthesised CtcelA gene Biomatik
 pBSK–cel6B Synthesised cel6B gene Biomatik
 pBSK–cglT Synthesised cglT gene Biomatik
 pBSK–CbcelA Synthesised CbcelA gene Biomatik
 pMTLgSlimS–LS3 G. thermoglucosidasius integration vector at pyrE locus Sheng et al. [26]
 pJ201–CtcelA Biobrick vector harbouring CtcelA gene flanked by ldh promoter This study
 pMTLgSlimS–CtcelA Expression vector for CtcelA with Pldh promoter This study
 pJ201–cel6B Biobrick vector harbouring cel6B gene preceded by Pldh promoter This study
 pMTLgSlimS–cel6B Expression vector for cel6B with Pldh promoter This study
 pJ201–CbcelA Biobrick vector harbouring CbcelA gene flanked by Pldh promoter This study
 pMTLgSlimS–CbcelA Expression vector for CbcelA with Pldh promoter This study
 pJ201–cglT Biobrick vector harbouring cglT gene flanked by Pldh promoter This study
 pMTLgSlimS–cglT Expression vector for cglT with Pldh promoter This study
 pMTLgSlimS–LS3–cglT Integration vector for CglT with Pldh promoter at pyrE locus This study
 pMTLgSlimS–CtcelAcel6B Expression vector for CtcelAcel6B operon with Pldh promoter This study