Table 1 Strains and plasmids used in this study

E. coli DH5α

supE44 ΔlacU169 (ϕ80lacZΔM15) hdR17 recA1 endA1 gyrA96 thi-1 relA1

[43]

S. cerevisiae strains

 Y294

α leu2-3,112 ura3-52 his3 trp1-289

ATCC 201160

 Y294[AmyA-GlaA]

URA3 ENO1_P-glaA-ENO1_T;

ENO1_P-amyA-ENO1_T

[19]

 Y294[GlaA-TemA]

URA3 ENO1_P-glaA-ENO1_T;

ENO1_P-temA-ENO1_T

This study

 Y294[TemG_Opt-AmyA]

URA3 ENO1_P-temG_Opt-ENO1_T;

ENO1_P-amyA-ENO1_T

This study

 Y294[TemG_Opt-AteA]

URA3 ENO1_P-temG_Opt-ENO1_T;

ENO1_P-ateA-ENO1_T

This study

 Y294[TemG_Opt-ApuA]

URA3 ENO1_P-temG_Opt-ENO1_T;

ENO1_P-apuA-ENO1_T

This study

 Y294[TemG_Opt-TemA]

URA3 ENO1_P-temG_Opt-ENO1_T;

ENO1_P-temA-ENO1_T

This study

 Y294[TemG_Opt-TemA_Opt]

URA3 ENO1_P-temG_Opt-ENO1_T;

ENO1_P-temA_Opt-ENO1_T

This study

 Y294[amdSYM]

URA3 TEF_P-amdS-TEF_T

This study

 ER^a

MATa/α prototroph

Fermentis, Lesaffre, France

 M2n

MATa/α prototroph

[16]

 ER T1^b

δ-integration of ENO1_P-temG_Opt-ENO1_T;

ENO1_P-temA-ENO1_T

This study

 ER T12^b

δ-integration of ENO1_P-temG_Opt-ENO1_T;

ENO1_P-temA-ENO1_T

This study

 M2n T1^b

δ-integration of ENO1_P-temG_Opt-ENO1_T;

ENO1_P-temA-ENO1_T

This study

 M2n T2^b

δ-integration of ENO1_P-temG_Opt-ENO1_T;

ENO1_P-temA-ENO1_T

This study

Plasmids

 yBBH1

bla URA3 ENO1_P-ENO1_T

[49]

 yBBH1-AmyA

bla URA3 ENO1_P-amyA-ENO1_T

[19]

 yBBH1-GlaA

bla URA3 ENO1_P-glaA-ENO1_T

[19]

 yBBH1-AteA

bla URA3 ENO1_P-ateA-ENO1_T

[20]

 yBBH1-ApuA

bla URA3 ENO1_P-apuA-ENO1_T

[20]

 yBBH1-TemA^c

bla URA3 ENO1_P-temA-ENO1_T

[20]

 yBBH1-TemA_Opt^c

bla URA3 ENO1_P-temA_Opt-ENO1_T

[20]

 yBBH1-TemG_Opt^d

bla URA3 ENO1_P-temG_Opt-ENO1_T

[20]

 yBBH1-TemG_Opt-TemA

bla URA3 ENO1_P-temG_Opt-ENO1_T;

ENO1_P-temA-ENO1_T

This study

 pUG-amdSYM^e

bla TEF1_P-amdS-TEF1_T

[17]

 yBBH1-amdSYM

bla URA3 TEF1_P-amdS-TEF1_T

This study