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Table 1 Strains and plasmids used in this study

From: Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch

Strains and plasmids Genotype References/source
E. coli DH5α supE44 ΔlacU169 (ϕ80lacZΔM15) hdR17 recA1 endA1 gyrA96 thi-1 relA1 [43]
S. cerevisiae strains
 Y294 α leu2-3,112 ura3-52 his3 trp1-289 ATCC 201160
 Y294[AmyA-GlaA] URA3 ENO1P-glaA-ENO1T;
ENO1P-amyA-ENO1T
[19]
 Y294[GlaA-TemA] URA3 ENO1P-glaA-ENO1T;
ENO1P-temA-ENO1T
This study
 Y294[TemG_Opt-AmyA] URA3 ENO1P-temG_Opt-ENO1T;
ENO1P-amyA-ENO1T
This study
 Y294[TemG_Opt-AteA] URA3 ENO1P-temG_Opt-ENO1T;
ENO1P-ateA-ENO1T
This study
 Y294[TemG_Opt-ApuA] URA3 ENO1P-temG_Opt-ENO1T;
ENO1P-apuA-ENO1T
This study
 Y294[TemG_Opt-TemA] URA3 ENO1P-temG_Opt-ENO1T;
ENO1P-temA-ENO1T
This study
 Y294[TemG_Opt-TemA_Opt] URA3 ENO1P-temG_Opt-ENO1T;
ENO1P-temA_Opt-ENO1T
This study
 Y294[amdSYM] URA3 TEFP-amdS-TEFT This study
 ERa MATa/α prototroph Fermentis, Lesaffre, France
 M2n MATa/α prototroph [16]
 ER T1b δ-integration of ENO1P-temG_Opt-ENO1T;
ENO1P-temA-ENO1T
This study
 ER T12b δ-integration of ENO1P-temG_Opt-ENO1T;
ENO1P-temA-ENO1T
This study
 M2n T1b δ-integration of ENO1P-temG_Opt-ENO1T;
ENO1P-temA-ENO1T
This study
 M2n T2b δ-integration of ENO1P-temG_Opt-ENO1T;
ENO1P-temA-ENO1T
This study
Plasmids
 yBBH1 bla URA3 ENO1P-ENO1T [49]
 yBBH1-AmyA bla URA3 ENO1P-amyA-ENO1T [19]
 yBBH1-GlaA bla URA3 ENO1P-glaA-ENO1T [19]
 yBBH1-AteA bla URA3 ENO1P-ateA-ENO1T [20]
 yBBH1-ApuA bla URA3 ENO1P-apuA-ENO1T [20]
 yBBH1-TemAc bla URA3 ENO1P-temA-ENO1T [20]
 yBBH1-TemA_Optc bla URA3 ENO1P-temA_Opt-ENO1T [20]
 yBBH1-TemG_Optd bla URA3 ENO1P-temG_Opt-ENO1T [20]
 yBBH1-TemG_Opt-TemA bla URA3 ENO1P-temG_Opt-ENO1T;
ENO1P-temA-ENO1T
This study
 pUG-amdSYMe bla TEF1P-amdS-TEF1T [17]
 yBBH1-amdSYM bla URA3 TEF1P-amdS-TEF1T This study
  1. aEthanol Red™ Version 1, referred to as ER
  2. bAmylolytic transformants contain integrated copies of ENO1P-temA-ENO1T and ENO1PtemG_Opt-ENO1T gene cassettes, the number indicates the transformant number during the screening process
  3. cAccession no. XM_013469492 for the native Talaromyces emersonii α-amylase (temA—1866 bp/622 amino acids)
  4. dAccession no. AJ304803 for the native T. emersonii glucoamylase (temG—1854 bp/618 amino acids)
  5. eAssession no. P30669 for pUG-amdSYM plasmid