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Table 3 PCR primers designed and used in this study with the relevant restriction sites underlined (XhoI = ctcgag, BamHI = ggatcc, BglII = agatct)

From: Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch

Primer name

Sequence (5′-3′)

ENOCASS-L

gtgcggtatttcacaccgcataggagatcgatcccaattaatgtgagttacctcactc

ENOCASS-R

cgggcctcttcgctattacgccagagcttagatct

amdSYMCas-L

ccgcgcgttggccgattcattaatccaggatccacatggaggcccagaataccctccttgac

amdSYMCas-R

gggcctcttcgctattacgccagagcttagatctcagtatagcgaccagcattcacatacttaa

Delta-ENO1_Promoter-L

tggaataaaaatccactatcgtctatcaactaatagttatattatcaatatattatcatatacggtgttaagatgatgacataagttatgagaagctgtcggatcccaattaatgtgagttacctcac

Delta-ENO1_Terminator-R

tgagatatatgtgggtaattagataattgttgggattccattgttgataaaggctataatattaggtatacagaatatactagaagttctcctcgaggatagatctcctatgcggtgtgaaataccgc