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Table 6 Product formation by S. cerevisiae ER and M2n strains after 192 h of fermentation at 30 °C and 37 °C in YPD media (containing 5 g l−1 glucose) and raw corn starch (200 g l−1), supplemented with selected GSHE dosages

From: Construction of industrial Saccharomyces cerevisiae strains for the efficient consolidated bioprocessing of raw starch

  30 °C 37 °C
S. cerevisiae strains ERa M2na ER T12 M2n T1 ER T12 M2n T1 ERa M2na ER T12 M2n T1 ER T12 M2n T1
GSHE added (µl) 28.3 28.3 2.8 2.8 0 0 28.3 28.3 2.8 2.8 0 0
Substrate (g l−1)
 Raw starch 200 200 200 200 200 200 200 200 200 200 200 200
 Glucose equivalent 208.5 208.5 208.5 208.5 208.5 208.5 208.5 208.5 208.5 208.5 208.5 208.5
Products (g l−1)
 Glucose 0.02 0.31 0.02 3.28 0.22 0.09 40.12 62.30 50.79 83.92 39.62 113.91
 Glycerol 4.07 4.30 4.76 4.59 3.18 3.73 5.72 5.64 6.07 5.54 5.50 2.63
 Acetic acid 0.00 0.00 0.90 0.31 0.64 0.00 0.83 0.66 0.95 0.39 1.28 0.95
 Ethanol 95.90 97.34 97.16 97.84 89.35 98.13 81.30 63.99 75.99 56.82 62.64 26.96
 Maltose 0.79 0.71 0.31 0.37 1.95 0.96 1.27 2.53 2.14 3.23 0.70 3.37
 CO2 91.73 93.11 92.93 93.59 85.46 93.87 77.76 61.21 72.69 54.35 59.92 25.79
Total 192.51 195.77 196.07 199.98 180.79 196.78 207.00 196.33 208.63 204.25 169.66 173.62
Estimated carbon conversion (%) 92 94 94 96 87 94 99 94 100 98 81 83
Ethanol yieldb (% of theoretical yield) 92 93 93 94 86 94 78 61 73 55 60 26
Ethanol productivityc 0.50 0.51 0.51 0.51 0.47 0.51 0.42 0.33 0.40 0.30 0.33 0.14
  1. aParental strains under SSF conditions
  2. bEthanol yield (% of the theoretical yield) was calculated as the amount of ethanol produced per gram of available glucose (at a specific time point)
  3. cEthanol productivity was calculated based on ethanol concentrations produced per hour (g l−1 h−1)