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Table 2 Yarrowia lipolytica strains and constructs used in this study

From: Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica

Strain/constructs name Genotype Description References
H222 SW1 H222 MATA ura3::suc2 Δku70 Improved homologous recombination capabilities [9]
H222 Δku70/CvFAP H222 MATA ura3::suc2 Δku70 including replicative plasmid p13001 (Additional file 1: Table S6) Production of CvFAP in strain background mentioned above This work
S33001 H222 Δpox1-6 alk1Δ::mTFP Δura3 Knocked out beta-oxidation for increased lipid accumulation and deleted ALK1 gene for reduced alkane degradation AG Kabisch, common strain collection
S33001/CvFAP H222 Δpox1-6 alk1Δ::mTFP Δura3 including replicative plasmid p13001 (Additional file 1: Table S6) Production of CvFAP in strain background mentioned above This work
S33001/empty vector control H222 Δpox1-6 alk1Δ::mTFP Δura3 including replicative plasmid p15018 (Additional file 1: Fig. S7) Strain background mentioned above, transformed with empty vector control This work
JMY5749 W29 Δmfe1 Δfaa1 pTEF-DGA2 pTEF-TGL4 pTEF-KlTGL3 FFA overproducing strain Referred as JMY5479 in [12]
JMY5749/CvFAP W29 Δmfe1 Δfaa1 pTEF-DGA2 pTEF-TGL4 pTEF-KlTGL3 including replicative plasmid p13001 (Additional file 1: Table S6) Production of CvFAP in FFA overproducing strain This work
JMY5749/empty vector control W29 Δmfe1 Δfaa1 pTEF-DGA2 pTEF-TGL4 pTEF-KlTGL3 including replicative plasmid p15018 (Additional file 1: Fig. S7) Strain background mentioned above, transformed with empty vector control This work
S07003 JMY5749 Δura3::54-55 Partial deletion of URA3 gene through a transformation with CRISPRyl-Hyg-URA3 This work
S07013 S07003 Δura3 alk1::pTEF1-CvFAP(WT) URA3 Integration of CvFAP cassette obtained from p13012 This work
S07004 S07003 Δura3 alk1::pTEF1-CvFAP(S121F) URA3 Integration of CvFAP cassette obtained from p13012 This work