Table 2 Yarrowia lipolytica strains and constructs used in this study

H222 SW1

H222 MATA ura3::suc2 Δku70

Improved homologous recombination capabilities

[9]

H222 Δku70/CvFAP

H222 MATA ura3::suc2 Δku70 including replicative plasmid p13001 (Additional file 1: Table S6)

Production of CvFAP in strain background mentioned above

This work

S33001

H222 Δpox1-6 alk1Δ::mTFP Δura3

Knocked out beta-oxidation for increased lipid accumulation and deleted ALK1 gene for reduced alkane degradation

AG Kabisch, common strain collection

S33001/CvFAP

H222 Δpox1-6 alk1Δ::mTFP Δura3 including replicative plasmid p13001 (Additional file 1: Table S6)

Production of CvFAP in strain background mentioned above

This work

S33001/empty vector control

H222 Δpox1-6 alk1Δ::mTFP Δura3 including replicative plasmid p15018 (Additional file 1: Fig. S7)

Strain background mentioned above, transformed with empty vector control

This work

JMY5749

W29 Δmfe1 Δfaa1 pTEF-DGA2 pTEF-TGL4 pTEF-KlTGL3

FFA overproducing strain

Referred as JMY5479 in [12]

JMY5749/CvFAP

W29 Δmfe1 Δfaa1 pTEF-DGA2 pTEF-TGL4 pTEF-KlTGL3 including replicative plasmid p13001 (Additional file 1: Table S6)

Production of CvFAP in FFA overproducing strain

This work

JMY5749/empty vector control

W29 Δmfe1 Δfaa1 pTEF-DGA2 pTEF-TGL4 pTEF-KlTGL3 including replicative plasmid p15018 (Additional file 1: Fig. S7)

Strain background mentioned above, transformed with empty vector control

This work

S07003

JMY5749 Δura3::54-55

Partial deletion of URA3 gene through a transformation with CRISPRyl-Hyg-URA3

This work

S07013

S07003 Δura3 alk1::pTEF1-CvFAP(WT) URA3

Integration of CvFAP cassette obtained from p13012

This work

S07004

S07003 Δura3 alk1::pTEF1-CvFAP(S121F) URA3

Integration of CvFAP cassette obtained from p13012

This work