Fig. 5

Time-course of chlorophyll a + b content and biomass (dry weight) concentration of E. gracilis in second culture of two-step E. gracilis culture systems. a Changes in chlorophyll a + b content in control culture of E. gracilis without co-culture with EG3 (black circle) and with co-culture with EG3 (white circle) in non-sterilized effluent during period of 7 days. b Changes in biomass concentration of E. gracilis in control culture without co-culture with EG3 (black circle) and with co-culture with EG3 (white circle) in non-sterilized effluent during period of 7 days. Values are means ± SDs (n = 3)