Strains | Genotype | Reference |
---|---|---|
4-JHS200 | JHS200, ura3Δ trp1Δ his3Δ leu2Δ | [19] |
4-PDC1d | 4-JHS200, pdc1Δ | In this study |
4-PDC5d | 4-JHS200, pdc5Δ | In this study |
4-PDC6d | 4-JHS200, pdc6Δ | In this study |
4-PDC16d | 4-JHS200, pdc1Δ pdc6Δ | In this study |
4-PDC56d | 4-JHS200, pdc5Δ pdc6Δ | In this study |
YG01 | 4-PDC16d, Δadh1::TDH3prom-Llnox-CYC1term | In this study |
YG01_SDB | YG01, p413_SDB | In this study |
YG01_SDBN | YG01, p413_SDB, p426TDH3_Llnox | In this study |
YG02 | 4-PDC56d, Δadh1::TDH3prom-Llnox-CYC1term | In this study |
YG02_SDB | YG02, p413_SDB | In this study |
Plasmids | Description | Reference |
---|---|---|
Cas9_Aur | p414-TEF1p-Cas9-CYC1t, modified Cas9 expression plasmid | [19] |
pRS42H | Backbone plasmid for constructing guideRNA plasmids | [40] |
gRNA_dPDC1 | pRS42H harboring PDC1 disruption gRNA cassette | In this study |
gRNA_dPDC5 | pRS42H harboring PDC5 disruption gRNA cassette | In this study |
gRNA_dPDC6 | pRS42H harboring PDC6 disruption gRNA cassette | In this study |
gRNA_dADH1 | pRS42H harboring ADH1 disruption gRNA cassette | In this study |
p413_SDB | 2,3-BDO biosynthesis pathway (plasmid p413 harboring alsS, alsD, BDH1) | [28] |
p426TDH3_Llnox | p426TDH3 harboring noxE gene from L. lactis and template of NADH oxidase expression cassette TDH3p-Llnox-CYC1t | [32] |