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Table 1 Strains and plasmids used in this study

From: Production of 2,3-butanediol from glucose and cassava hydrolysates by metabolically engineered industrial polyploid Saccharomyces cerevisiae

Strains

Genotype

Reference

4-JHS200

JHS200, ura3Δ trp1Δ his3Δ leu2Δ

[19]

4-PDC1d

4-JHS200, pdc1Δ

In this study

4-PDC5d

4-JHS200, pdc5Δ

In this study

4-PDC6d

4-JHS200, pdc6Δ

In this study

4-PDC16d

4-JHS200, pdc1Δ pdc6Δ

In this study

4-PDC56d

4-JHS200, pdc5Δ pdc6Δ

In this study

YG01

4-PDC16d, Δadh1::TDH3prom-Llnox-CYC1term

In this study

YG01_SDB

YG01, p413_SDB

In this study

YG01_SDBN

YG01, p413_SDB, p426TDH3_Llnox

In this study

YG02

4-PDC56d, Δadh1::TDH3prom-Llnox-CYC1term

In this study

YG02_SDB

YG02, p413_SDB

In this study

Plasmids

Description

Reference

Cas9_Aur

p414-TEF1p-Cas9-CYC1t, modified Cas9 expression plasmid

[19]

pRS42H

Backbone plasmid for constructing guideRNA plasmids

[40]

gRNA_dPDC1

pRS42H harboring PDC1 disruption gRNA cassette

In this study

gRNA_dPDC5

pRS42H harboring PDC5 disruption gRNA cassette

In this study

gRNA_dPDC6

pRS42H harboring PDC6 disruption gRNA cassette

In this study

gRNA_dADH1

pRS42H harboring ADH1 disruption gRNA cassette

In this study

p413_SDB

2,3-BDO biosynthesis pathway (plasmid p413 harboring alsS, alsD, BDH1)

[28]

p426TDH3_Llnox

p426TDH3 harboring noxE gene from L. lactis and template of NADH oxidase expression cassette

TDH3p-Llnox-CYC1t

[32]