Table 1 Bacterial strains and plasmids used in this study
Name | Description | Reference |
|---|---|---|
Strains | ||
 DH5α |  | RBC |
 Wild-type | BL21(DE3) | KCTC |
 EM | BL21(DE3) harboring pZAM | This study |
 EMK | EM harboring pZAM01 | This study |
 EMK00 | EMK ΔgcvP | This study |
 EMK01 | EMK00 harboring pZAM02 | This study |
 EMK02 | EMK00 harboring pZAM02 and pCDM02 | This study |
 EMK02A2 | ALE mutant from EMK02 for 60th serial subcultures | This study |
 EMK02A5 | ALE mutant from EMK02 for 150th serial subcultures | This study |
 vA5f | EMK02A2 harboring pZSfimCD | This study |
 vA5y | EMK02A2 harboring pZSydeH | This study |
 vA5h | EMK02A2 harboring pZShtrE | This study |
 vA5c | EMK02A2 harboring pZScsgD | This study |
 EMK02etOH | EMK02 harboring pZSetOH | This study |
 EMK02A2etOH | EMK02A2 harboring pZSetOH | This study |
 EMK02A5etOH | EMK02A5 harboring pZSetOH | This study |
Plasmids | ||
 pZA31MCS | p15A ori PLtetO-1 CmR | Expressys |
 pZS21MCS | pCS101 ori PLtetO-1 KanR | Expressys |
 pCDFDuet-1 | Double T7 promoter, CloDF13 ori, SmR | Novagen |
 pKD4 | FRT flanked resistance cassette involved vector, oriRγ, KmR | Datsenko and Wanner [42] |
 pRedET | Red recombinase expression plasmid, repA, pSC101ori, PBAD, gam, beta alpha, recA, tetR | Gene Bridge |
 707FLP | Flippase expression plasmid, pSC101ori, repA, cl578, FLPe, tetR | Gene Bridge |
 pZAM | pZA31MCS containing gene ftfL from M. extorquens AM1 | This study |
 pZAM01 | pZAM containing genes mtdA and fch from M. extorquens AM1 | This study |
 pZAM02 | pZAM01 containing gene glyA from M. extorquens AM1 | This study |
 pCDM02 | pCDFDuet-1 containing genes sga, hpr and gck from M. extorquens AM1 | This study |
 pZSetOH | pZS21MCS containing genes pdc from Z. mobilis and adhA from L. lactis | This study |
 pZSfimCD | pZS21MCS containing genes fimC and fimD from E. coli | This study |
 pZSydeH | pZS21MCS containing gene ydeH from E. coli | This study |
 pZShtrE | pZS21MCS containing gene htrE from E. coli | This study |
 pZScsgD | pZS21MCS containing gene csgD from E. coli | This study |