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Table 1 Bacterial strains and plasmids used in this study

From: Adaptively evolved Escherichia coli for improved ability of formate utilization as a carbon source in sugar-free conditions

Name Description Reference
Strains
 DH5α   RBC
 Wild-type BL21(DE3) KCTC
 EM BL21(DE3) harboring pZAM This study
 EMK EM harboring pZAM01 This study
 EMK00 EMK ΔgcvP This study
 EMK01 EMK00 harboring pZAM02 This study
 EMK02 EMK00 harboring pZAM02 and pCDM02 This study
 EMK02A2 ALE mutant from EMK02 for 60th serial subcultures This study
 EMK02A5 ALE mutant from EMK02 for 150th serial subcultures This study
 vA5f EMK02A2 harboring pZSfimCD This study
 vA5y EMK02A2 harboring pZSydeH This study
 vA5h EMK02A2 harboring pZShtrE This study
 vA5c EMK02A2 harboring pZScsgD This study
 EMK02etOH EMK02 harboring pZSetOH This study
 EMK02A2etOH EMK02A2 harboring pZSetOH This study
 EMK02A5etOH EMK02A5 harboring pZSetOH This study
Plasmids
 pZA31MCS p15A ori PLtetO-1 CmR Expressys
 pZS21MCS pCS101 ori PLtetO-1 KanR Expressys
 pCDFDuet-1 Double T7 promoter, CloDF13 ori, SmR Novagen
 pKD4 FRT flanked resistance cassette involved vector, oriRγ, KmR Datsenko and Wanner [42]
 pRedET Red recombinase expression plasmid, repA, pSC101ori, PBAD, gam, beta alpha, recA, tetR Gene Bridge
 707FLP Flippase expression plasmid, pSC101ori, repA, cl578, FLPe, tetR Gene Bridge
 pZAM pZA31MCS containing gene ftfL from M. extorquens AM1 This study
 pZAM01 pZAM containing genes mtdA and fch from M. extorquens AM1 This study
 pZAM02 pZAM01 containing gene glyA from M. extorquens AM1 This study
 pCDM02 pCDFDuet-1 containing genes sga, hpr and gck from M. extorquens AM1 This study
 pZSetOH pZS21MCS containing genes pdc from Z. mobilis and adhA from L. lactis This study
 pZSfimCD pZS21MCS containing genes fimC and fimD from E. coli This study
 pZSydeH pZS21MCS containing gene ydeH from E. coli This study
 pZShtrE pZS21MCS containing gene htrE from E. coli This study
 pZScsgD pZS21MCS containing gene csgD from E. coli This study